Laboratory Services

Laboratory

Diseases

Test types

Remarks

Collaboratio

with Int.

Bacterial Serology 

Brucellosis

  • Rose Bengal Plate Test (RBPT)
  •  Latex Agglutination test
  • Complement FixationTests (CFT)

RBPT Recommended for
accreditation ISO 17025; yearly participate on Proficiency Test

Contagious Bovine
Pleuropneumonia (CBPP)

  •  cELISA

CIRAD

Contagious Caprine
Pleuropneumonia (CBPP)

  •  cELISA

Cell Culture and Virus Isolation

Multiple

  • Isolation and identification ofviruses(PPR,AHS,LSD,RP, Sheep box)
  • Detection of virus antigens
  • Virus/serum neutralizatio tests
  •   IFAT


LSD and Capripox

CIRAD and IAH, Pirbright, UK

For AHS, BVDV, CSF


IAH, Pirbright, UK

Viral Serology1

FMD

  •  3ABC  ELISA, Liquid Phase Blocking ELISA; Core and surface antibody detection

3ABC ELISA recommended
for Accreditation ISO/IEC 17025 yearly participate on Proficiency Test

IAH, Pirbright, UK

PPR,

  •  C-ELISA, Ag  detection ELISA; Both Ab and Ag detection:

PPR ELISA is
recommended for Accreditation ISO/IEC 17025 yearly participate on Proficiency Test

CIRAD, France and IAEA, Austria

Maedi-Visna and BVD

  •  ELISA

Viral Serology2

Pathology

RVF

 

  • Inhibition-IgM-and_IgG
  • ELISAs

recommended for
Accreditation ISO/IEC 17025 yearly participate on Proficiency Test

ARC, OVI, S. Africa

Gumboro, Newcastle,
African Horse Sickness

  •  ELISA

AHS and  EEV

with IAH, Pirbright,UK

Avian Influenza

  •  AGID

yearly participate on Proficiency Test

NDV and AI,

VLA, UK and

IZSVe,Padova,

Italy

Others

  •  Toxoplasmosis, Bluetongue

Based on Request

EHV with VLA

Multiple

  •  Usually Hematoxylin and Eosin staining method.
  • Others like Ziehl-Neelsen (Acid fast) stain, Van Gieson.s collagen Fiber stain, Kossa’s staining method for calcium and inclusion bodies etc are also used when necessary

With Good Facility for
Postmortem and Necropsy Investigation

Pharmacology

  •  Mostly research on Ethno-veterinary following Herbal medicines used by livestock owners

Makes ready for invivo
and Invitro trial

Acarology, Entimology nd Helmintology

Different
ecto-parasites

  •  Taxonomiciden tification of different ectoparasites
  •  Acaricide resistance testing
  •  Verification of the efficacy of acaricides

Laboratory rearing of
major economically important ticks for diagnostic, research, acaricide
testing, control testing and for teaching.

Helminthes

  •  Coprology, Larval Identification and Antihelmentics Resistance study
  •   In vivo and in vitro testing of plant extracts for the control of Ecto-and-Endo-parasites

Trails on three plants
reached scaling up

Protozoology

Hemoparasites

  •  Serological tests for Trypanosomosis, ECF and tick-borne diseases.
  •  Smearfor hemoparasites
  •  Buffy coat technique for Trypanosomosis.

Research on
Trypanotolerant breeds in Ethiopia successfully completed

Biosafety Level 3 Avian Flu and Zoonoses
(Airflow is calibrated)

Avian/poultry Diseases

  •  Virus Isolation on Embryonated Chicken eggs

A lot of them isolated

NDV, AI and PPMV1

  •  Hemagglutination and Hemagglutination Inhibition tests

yearly participate on Proficiency Test;

VLA and IAH,
Pirbright, UK

Sample Reception Unit

All diseases

  • Continuously Serve on receiving Samples from customers, gives unique code to each sample, dispatch to the labs based on customers demand and compile and send results to customers

Fulfills ISO/IEC 17025: 2005 requirements

General Bacteriology and Mycology

Multiple, Major Bacterial and fungal diseases of animals

  •  Aerobic and anaerobic bacteria culture and identification
  •  Filamentous fungus and yeast culture and identification
  • Bacterial and fungal suspected colonies can be identified using primary and secondary biochemical techniques, API kits and OMNILOG/BIOLOG bacterial identification technique
  •  Antimicrobial susceptibility test can be conducted using disc diffusion test method

 

 

Bovine tuberculosis

  •  Comparative intradermal tuberculin test (CIDT)
  •  Mycobacterium bovis culture and identification

 

Culture of M. bovis from positive animals can be carried for research purpose at BSL III laboratory

Currently under ETHICOBOTS

Project with

Cambridge

University, UK https://

ethicobots.com

Molecular Diagnosis

 

NDV, Avian Influenza, Infectious Bursal Diseases, Marek’s disease, RVF ,FMD, ASF , CBPP, Brucellosis , Sheep & goat Pox , LSD , PPR , MERS .

  •  Both conventional & Real- time PCR

 


Accredited PCR test for AI &NDV  by the requirement of   ISO/IEC 17025

yearly participate on Proficiency Test and Offlu ring trial tests

 

 

PADOVA, Italy

Avian Influenza and NDV

  •  Egg inoculation and Heamaglutination  tests

Accredited by the requirement of   ISO/IEC 17025

yearly participate on Proficiency Test

 

PADOVA ,Italy