Mr. George MAGORA from Ministry of Agriculture and Rural Development, Department of Veterinary services of Zimbabwe recently completed a one month fellowship in Ethiopia from 1st to 30th September 2016. The fellowship was hosted by National Animal Health Diagnostic and Investigation Center (NAHDIC) under the supervision of Dr Tesfaye Rufael Chibssa Tel. +251 911764972; Email:chibssasafo@gmail.com. The training was related with TC Project ZIM/5/022 on Tissue Culture techniques to establish Surveillance of Livestock Diseases in Zimbabwe.

The fellowship focused on Cell culture and emphasized training in laboratory techniques.
 Among the laboratory techniques addressed were:

  1. Basic Cell culture technology; Preparation of cell growth medium for cell lines growth, microscopically checking of the Cells to ensure healthy and growing as expected, Sub-culturing and Cell feeding for cell culture maintenance, freezing of harvested cells in liquid nitrogen until ready to use, thawing and culturing of cells and Cell Counts to determine the cell density of the culture.
  2. Virus isolation; Pathological samples for Capripox virus, PPR and FMD was collected and inoculated on Vero cells and cytopathic effects (CPE) was observed for the virus growth.The isolated virus was farther tested by using virus neutralization tests (VNT) and immumofluorence antibody tests (IFAT) for diagnosis of viral diseases.
  3. Quality Control Considerations was also emphasized during the training in all aspects of tissue culture. The main areas specified for quality control in tissue culture were quality of materials used (cell lines, media and other reagents) and personal practices for avoidance of microbial contamination. The need of Certificates of Analysis and Safety Data sheet from Manufacturers during supply of reagents and materials for tissue culture use were explained. The training was comprehensive and included work with experimental animals in addition to laboratory analysis.

The trainee derives the maximum benefit from training courses and the techniques taught  will be applicable in Zimbabwe for establishing of Tissue Culture techniques for the Surveillance of Livestock Diseases. If successful, this work will benefit the local farmers, allowing them to increase the productivity of their animal and the income resulting from their farming systems.

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